Difference between revisions of "Part:BBa K875001"

Revision as of 14:15, 23 September 2012

T5 Cumate Operator

This promoter is composed by phage T5 promoter and Cumate operator. It is repressed in the presence of CymR protein which binds the Cumate Operator.

1. The cumate gene-switch: a system for regulated expression in mammalian cells Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )

2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains Young J. Choi, Lyne Morel, Teffanie Le François Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. Míguez (Applied and Environmental Microbiology, Aug. 2010, p. 5058–5066 Vol.76 doi:10.1128/AEM.00413-10)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K875001 short</partinfo>
-&#65532;&#65532;&#65532;This promoter is composed by phage T5 promoter and Cumate operator. It is repressed in the  presence of CymR protein which binds the Cumate Operator.
+&#65532;&#65532;&#65532;
+This promoter is composed by phage T5 promoter and Cumate operator. It is repressed in the  presence of CymR protein which binds the Cumate Operator.
+. The cumate gene-switch: a system for regulated expression in mammalian cells
+Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43   doi:10.1186/1472-6750-6-43 )
+. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains                  Young J. Choi, Lyne Morel, Teffanie Le François Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. Míguez (Applied and Environmental Microbiology, Aug. 2010, p. 5058–5066 Vol.76   doi:10.1128/AEM.00413-10)
+[[Image:Cumts3.png|center|500px]]
 <!-- Add more about the biology of this part here