Difference between revisions of "Part:BBa K733009"

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Revision as of 14:09, 23 September 2012

Ptms+BBa_E0240: Ptms+RBS+GFP+Double

pTms is a promoter which proves to be functional in both E. coli and B. subilis. R.P.U standard is advocated by part registry to represent the efficiency of a constitutive promoter.(Kelly et al., 2009) We intend to characterize promoter pTms in E. coli. Using E. coli as our initial characterization chassis may prove to be more valuable for most of iGEM participants, because E. coli is frequently used by iGEM participants.

Link to our pTms promoter: BBa_K733001

Link with our characterization page.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736


Reference

Kelly J, Rubin A, Davis J, Ajo-Franklin C, Cumbers J, Czar M, de Mora K, Glieberman A, Monie D, Endy D: Measuring the activity of BioBrick promoters using an in vivo reference standard. Journal of Biological Engineering 2009, 3:4.