Difference between revisions of "Part:BBa K808032:Design"

 
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===References===
 
===References===
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* Yang, T. H., Pan, J. G., Seo, Y. S., & Rhee, J. S. (2004). Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli, 70(12), 6968–6976. doi:10.1128/AEM.70.12.6968
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* Fakult, M., & Enzymtechnologie, M. (2006). biochemische Charakterisierung Dialkylphthalat spaltender Esterasen.
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* Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177–82. doi:10.1016/j.febslet.2004.12.087

Latest revision as of 13:56, 23 September 2012

Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2392
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2918
    Illegal NgoMIV site found at 3056
    Illegal NgoMIV site found at 3596
    Illegal NgoMIV site found at 3965
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2463
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence.


Source

assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector)

References

  • Yang, T. H., Pan, J. G., Seo, Y. S., & Rhee, J. S. (2004). Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli, 70(12), 6968–6976. doi:10.1128/AEM.70.12.6968
  • Fakult, M., & Enzymtechnologie, M. (2006). biochemische Charakterisierung Dialkylphthalat spaltender Esterasen.
  • Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177–82. doi:10.1016/j.febslet.2004.12.087