Difference between revisions of "Part:BBa K743008"

Revision as of 13:46, 23 September 2012

psb1C3_IntKR recombination plasmid for Synechocystis PCC6803

This plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly between RS1 and B0015. As synechocystis naturally undergoes double homologous recombination, any sequence between RS1 and RS2 will be introduced in to it´s chromosome along with a KanR gene for selection in cyanobacteria or in E.coli The plasmid backbone also has a Chloramphenicol resistance casette for selection in E.coli only. The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.

It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1758
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1758
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1758
Illegal XhoI site found at 2255
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1758
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1758
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 511

Verification Colony PCR

psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1

@@ Line 7: / Line 7: @@
 It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis
-[[Image:gel_UC_Chile.jpg|400px]]
-'''Verification Colony PCR'''
-[[Image:gel_uc_chile.jpg|400px]]
-'''psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1'''
 <!-- Add more about the biology of this part here
@@ Line 20: / Line 14: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K743008 SequenceAndFeatures</partinfo>
+[[Image:gel_UC_Chile.jpg|400px]]
+'''Verification Colony PCR'''
+[[Image:gel_uc_chile.jpg|400px]]
+'''psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1'''