Difference between revisions of "Part:BBa K875001:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | = | + | '''Trieste Team 2012''' |
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| + | In liquid assay: | ||
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| + | We inoculated a 20ml culture. After overnight growth, we diluted the culture to OD600 = 0.2. Then we aliquoted 200 μl in 8 replicates in a microtiter plate at different concentrations of cumate. The reading was performed in a monochromator at 485-510nm. | ||
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| + | [[Image:T5opTS.png|frame|center|600px|alt="Graph 1"]] | ||
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| + | In the plate assay: | ||
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| + | We streaked the culture on LB Agar plates containing different p-cumate concentrations. | ||
| + | |||
| + | [[Image:T5op2ts.png|frame|center|20px|alt="pic 1"]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:41, 23 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Trieste Team 2012
In liquid assay:
We inoculated a 20ml culture. After overnight growth, we diluted the culture to OD600 = 0.2. Then we aliquoted 200 μl in 8 replicates in a microtiter plate at different concentrations of cumate. The reading was performed in a monochromator at 485-510nm.
In the plate assay:
We streaked the culture on LB Agar plates containing different p-cumate concentrations.
User Reviews
UNIQ4a173e94602921be-partinfo-00000000-QINU UNIQ4a173e94602921be-partinfo-00000001-QINU