Difference between revisions of "Part:BBa K909011"

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The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.
 
The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.
  
[[Image: tetlac.pdf]]
+
[[Image: tetlac.jpeg]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:30, 23 September 2012

Dual input promoter: PL promoter with two operator sites TetO1 and LacO1

The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.

File:Tetlac.jpeg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]