Difference between revisions of "Part:BBa K909011"
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The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2. | The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2. | ||
+ | |||
+ | LacI TetR Output | ||
+ | + + - | ||
+ | + - - | ||
+ | - + - | ||
+ | - - + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 13:22, 23 September 2012
Dual input promoter: PL promoter with two operator sites TetO1 and LacO1
The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.
LacI TetR Output + + - + - - - + - - - +
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]