Difference between revisions of "Part:BBa K909011"

Line 3: Line 3:
  
 
The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.
 
The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.
 +
 +
LacI TetR Output
 +
+ + -
 +
+ - -
 +
- + -
 +
- - +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:22, 23 September 2012

Dual input promoter: PL promoter with two operator sites TetO1 and LacO1

The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators TetO1 and LacO1 and LacO2.

LacI TetR Output + + - + - - - + - - - +

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]