Difference between revisions of "Part:BBa K861178"
 
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<partinfo>BBa_K861178 short</partinfo>
 
<partinfo>BBa_K861178 short</partinfo>
  
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In this device, a constitutively expressed CRP is assembled with promoter Pcar(BBa_K861171), hoping to make the promoter less leaky. RFP was used as a reporter.
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In this device, a constitutively expressed CRP is assembled with promoter Pcar with RFP(BBa_K861176), hoping to see the effect of CRP on the function of Pcar. RFP was used as a reporter.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:59, 22 September 2012

Pcar efficacy testing device with CRP overexpressed

In this device, a constitutively expressed CRP is assembled with promoter Pcar with RFP(BBa_K861176), hoping to see the effect of CRP on the function of Pcar. RFP was used as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 815
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1425
    Illegal AgeI site found at 1537
  • 1000
    COMPATIBLE WITH RFC[1000]