Difference between revisions of "Part:BBa K861174"
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<partinfo>BBa_K861174 short</partinfo>
 
<partinfo>BBa_K861174 short</partinfo>
  
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The promoter, BBa_J23113, was used to constitutively express cI protein (BBa_P0451). Promoter lambda - cI regulated (BBa_R0051) with RFP (BBa_E1010) was used as a reporter.  
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This is an intermediate device for function detection of the indirect regulatory device K861169. In the device, a constitutively expressed cI is assembled with promoter repressed by cI, resulting in repression of the gene downstream. In comparison with the device K861169 which is activated by glucose, RFP in this device will not be expressed at any glucose concentration.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 18:31, 22 September 2012

A control device for BBa_K861169

This is an intermediate device for function detection of the indirect regulatory device K861169. In the device, a constitutively expressed cI is assembled with promoter repressed by cI, resulting in repression of the gene downstream. In comparison with the device K861169 which is activated by glucose, RFP in this device will not be expressed at any glucose concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1611
    Illegal AgeI site found at 1723
  • 1000
    COMPATIBLE WITH RFC[1000]