Difference between revisions of "Part:BBa K733018"

 
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<partinfo>BBa_K733018 short</partinfo>
 
<partinfo>BBa_K733018 short</partinfo>
  
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This construct is built to measure the relative efficiency of xylose inducible promoter in E. coli. We intend to use GFP as an indicator to characterize this xylose inducible promoter derived from Bacillus megaterium ([https://parts.igem.org/Part:BBa_K733002 BBa_K733002]). In our characterization, we will investigate the influence of concentration of xylose on xylose inducible promoter and use GFP intensity changes over certain period of time to represent the regulatory effect of this promoter in different xylose inducing conditions.
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'''Link: our characterization.'''
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:51, 22 September 2012

xylR+PxylA+RBS+GFP+Double Terminator

This construct is built to measure the relative efficiency of xylose inducible promoter in E. coli. We intend to use GFP as an indicator to characterize this xylose inducible promoter derived from Bacillus megaterium (BBa_K733002). In our characterization, we will investigate the influence of concentration of xylose on xylose inducible promoter and use GFP intensity changes over certain period of time to represent the regulatory effect of this promoter in different xylose inducing conditions.

Link: our characterization.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2058