Difference between revisions of "Part:BBa K808000"

Revision as of 16:55, 22 September 2012

araC-Pbad - Arabinose inducible regulatory promoter/repressor unit

This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter. L(+) - Arabinose is a sugar and is harmless. For overexpression of proteins the concentration in range of 0,001-0,02% Arabinose can be used.

This sequenced biobrick was alingned by using nucleotide NCBI BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_SPEC=WGS&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch) with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.

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 __NOTOC__
 <partinfo>BBa_K808000 short</partinfo>
-This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
+This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter. L(+) - Arabinose is a sugar and is harmless.
-<span class='h3bb'>Sequence and Features</span>
+For overexpression of proteins the concentration in range of 0,001-0,02% Arabinose can be used.
-<partinfo>BBa_K808000 SequenceAndFeatures</partinfo>
+This sequenced biobrick  was alingned by using nucleotide NCBI BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_SPEC=WGS&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch) with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K808000 parameters</partinfo>
-<!-- -->
+<!-- →