Difference between revisions of "Part:BBa K861400"
 
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For E.coli to degrade fatty acid, at least four enzymes – FadA, FadB, FadD, FadE and transporter—FadL are needed. Long chain fatty acids(FAs) are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthetase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The following cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.
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For E.coli to degrade fatty acid, at least four enzymes – FadA, FadB, FadD, FadE and a transporter—FadL are needed. Long chain fatty acids(FAs) are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthetase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The following cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.
  
 
<partinfo>BBa_K861400 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K861400 SequenceAndFeatures</partinfo>

Latest revision as of 15:37, 22 September 2012

For E.coli to degrade fatty acid, at least four enzymes – FadA, FadB, FadD, FadE and a transporter—FadL are needed. Long chain fatty acids(FAs) are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthetase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The following cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BglII site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 574
  • 1000
    COMPATIBLE WITH RFC[1000]