Difference between revisions of "Part:BBa K861170"

Latest revision as of 15:30, 22 September 2012

PI ,Glucose-activated promoter

This is a promoter designed for the Eschaerichia coli. It contains the altered consensus CRP-binding site and the consensus RNA polymerase-binding site which have an overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cells. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to Pcar (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K861170 short</partinfo>
-This is a promoter designed for the Eschaerichia coli. It contains the altered consensus CRP-binding site and the consensus RNA polymerase-binding site which have an overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration  will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to Pcar (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter.
+This is a promoter designed for the Eschaerichia coli. It contains the altered consensus CRP-binding site and the consensus RNA polymerase-binding site which have an overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration  will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cells. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to Pcar (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter.
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