Difference between revisions of "Part:BBa K896007"
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[[Image:Nap cloning.jpg]]
 
[[Image:Nap cloning.jpg]]
  
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The Prokaryotic Nitrate Reductases
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David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson
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(David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson, The Prokaryotic Nitrate Reductases)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:07, 22 September 2012

Nap( Nitrate->Nitrite reductase)

A type of respiratory NO3-reductase is a water-soluble, periplasmicallylocated protein known as Nap. We acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774, the function of Nap is to transfer NO into nitrogen.

Nap cloning.jpg


(David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson, The Prokaryotic Nitrate Reductases)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1594
    Illegal BamHI site found at 1803
    Illegal XhoI site found at 2361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
    Illegal NgoMIV site found at 2983
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1342
    Illegal SapI.rc site found at 1903