Difference between revisions of "Part:BBa K743009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This composite part was assembled by Gibson assembly technique. | |
| − | + | It is designed to be edited by Gibson assembly, and not by standard biobrick assembly, because of a EcoR1 restriction site present in [BBa_K743001 RS2 recombination sequence] | |
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===Source=== | ===Source=== | ||
Revision as of 14:46, 22 September 2012
psb1C3_IntKR recombination plasmid with RFP under psaAB promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2657
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2657
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2657
Illegal XhoI site found at 3154 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2657
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2657
Illegal AgeI site found at 1270
Illegal AgeI site found at 1382 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
This composite part was assembled by Gibson assembly technique. It is designed to be edited by Gibson assembly, and not by standard biobrick assembly, because of a EcoR1 restriction site present in [BBa_K743001 RS2 recombination sequence]
Source
Go to https://parts.igem.org/Part:BBa_K743008 for information about the plasmid backbone. Both the psaAB promoter and the RFP coding sequence were obtained from the registry.