Difference between revisions of "Part:BBa K861038"

Latest revision as of 14:13, 22 September 2012

IPTG induced S-FadA and S-FadB

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadA and FadB protein in Salmonella enterica LT2.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 168
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2391
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1621

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K861038 short</partinfo>
-To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadA and FadB protein in <i>Salmonella enterica LT2</i>.
+To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadA and FadB protein in <i>Salmonella enterica LT2</i>.
 <!-- Add more about the biology of this part here