Difference between revisions of "Part:BBa K911001:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K911001=== | ===Applications of BBa_K911001=== | ||
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| + | BBa_K911001 has been inserted into the following construct: | ||
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| + | [[Image:MgConstruct1.jpg]] | ||
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| + | The sequence of this construct has been verified. | ||
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| + | In order to test it, we transformed the construct into e.coli and attempted to induce GFP expression with both increasing magnesium concentrations and increasing IPTG concentrations. The cells were then interrogated at the correct GFP absorbtion and emission wavelengths (450nm and 545nm respectively) in a plate reader. | ||
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| + | The results of this assay are shown below. | ||
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| + | [[Image:MgEcoliAssay.jpg]] | ||
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| + | As can be seen, the assay did not give the expected results. Neither IPTG nor magnesium appeared to alter the expression of sfGFP. | ||
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| + | The construct was then transformed into Bacillus subtilis, with a view to repeat the same assay in this new chassis. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:07, 22 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K911001
BBa_K911001 has been inserted into the following construct:
The sequence of this construct has been verified.
In order to test it, we transformed the construct into e.coli and attempted to induce GFP expression with both increasing magnesium concentrations and increasing IPTG concentrations. The cells were then interrogated at the correct GFP absorbtion and emission wavelengths (450nm and 545nm respectively) in a plate reader.
The results of this assay are shown below.
As can be seen, the assay did not give the expected results. Neither IPTG nor magnesium appeared to alter the expression of sfGFP.
The construct was then transformed into Bacillus subtilis, with a view to repeat the same assay in this new chassis.
User Reviews
UNIQ802258cff3d5cb5d-partinfo-00000000-QINU UNIQ802258cff3d5cb5d-partinfo-00000001-QINU