Difference between revisions of "Part:BBa K861028"
 
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<partinfo>BBa_K861028 short</partinfo>
 
<partinfo>BBa_K861028 short</partinfo>
  
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The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE. Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. We assembled FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.  
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The first step in the beta-oxidation pathway involves the conversion of acyl-CoA into enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE. Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. We assembled FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.  
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 14:01, 22 September 2012

PfadR regulated FadE

The first step in the beta-oxidation pathway involves the conversion of acyl-CoA into enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE. Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. We assembled FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 472
    Illegal SapI.rc site found at 1345
    Illegal SapI.rc site found at 2488