Difference between revisions of "Part:BBa K861346"
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<partinfo>BBa_K861346 short</partinfo> | <partinfo>BBa_K861346 short</partinfo> | ||
− | To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different | + | To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This is an intermediate part for BBa_K861036. |
Latest revision as of 12:54, 22 September 2012
IPTG induced promoter with FadA, intermediate part for BBa_K861036
To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This is an intermediate part for BBa_K861036.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 168
Illegal BglII site found at 1044 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 658
- 1000COMPATIBLE WITH RFC[1000]