Difference between revisions of "Part:BBa K896006"

Revision as of 12:53, 22 September 2012

NirN-NirS (Nitrite->Nitric oxide reductase)

Cloning of SQR gene:

SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1703
Illegal BamHI site found at 1483
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 52
Illegal NgoMIV site found at 168
Illegal NgoMIV site found at 220
Illegal NgoMIV site found at 604
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 2305
Illegal AgeI site found at 3169
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2347
Illegal BsaI.rc site found at 2479

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K896006 short</partinfo>
-YES
+Cloning of SQR gene:
+SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.<p></p>
+[[Image:NirN+S cloning.jpg]]
 <!-- Add more about the biology of this part here