Difference between revisions of "Part:BBa K861070"

Revision as of 08:27, 22 September 2012

AdrA gene from E.coli K12

This is the coding sequence of gene AdrA, which contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. The gene can not be cloned using the standard provided by the registry. We therefore use the primer in the paper(Davide Antoniani et al. Appl Microbiol Biotechnol.2009) to clone this gene. This will lead to about 40bp gemone sequnce added upstream the start codon.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 523
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 08:23, 22 September 2012 (view source) Shinkwee (Talk \| contribs) ← Older edit		Revision as of 08:27, 22 September 2012 (view source) Shinkwee (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K861070 short</partinfo>		<partinfo>BBa_K861070 short</partinfo>

−	This is the coding sequence of gene AdrA, which contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. The gene can not be cloned using the standard provided by the registry. We therefore use the primer in the paper(Davide Antoniani et al. Appl Microbiol Biotechnol.09) to clone this gene. This will lead to about 40bp gemone sequnce added upstream the start codon.	+	This is the coding sequence of gene AdrA, which contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. The gene can not be cloned using the standard provided by the registry. We therefore use the primer in the paper(Davide Antoniani et al. Appl Microbiol Biotechnol.2009) to clone this gene. This will lead to about 40bp gemone sequnce added upstream the start codon.