Difference between revisions of "Part:BBa K861016"
 
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<partinfo>BBa_K861016 short</partinfo>
 
<partinfo>BBa_K861016 short</partinfo>
  
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In Escherichia coli, after fatty acids being transported into the cell, to be degraded, they must be activated by the acyl-CoA synthetase-- FadD (BBa_K861010) to form CoA derivatives. Also, FadD is shown to be essential for fatty acids transportation. We assembled FadD with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.  
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In E.coli, after fatty acids being transported into the cell, to be degraded, they must be activated by the acyl-CoA synthetase-- FadD (BBa_K861010) to form CoA derivatives. Also, FadD is shown to be essential for fatty acids transportation. We assembled FadD with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.  
  
  

Latest revision as of 07:54, 22 September 2012

PfadR regulated FadD

In E.coli, after fatty acids being transported into the cell, to be degraded, they must be activated by the acyl-CoA synthetase-- FadD (BBa_K861010) to form CoA derivatives. Also, FadD is shown to be essential for fatty acids transportation. We assembled FadD with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]