Difference between revisions of "Part:BBa K907002"

Revision as of 07:30, 22 September 2012

Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS

Dual phase protein generator, RBS(reverse) - attB - Promoter - attP - RBS 600px

This part is composed of 3 elements.
1. RBS: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.

At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).

This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N

Sequence and Features