Difference between revisions of "Part:BBa K861003"

Revision as of 06:53, 22 September 2012

PfadR regulated FadL

Long-chain fatty acids are transported across the cell membrane via a transportation/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The fadL gene codes for a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transportation. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assembled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1207
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K861003 short</partinfo>
-Long-chain fatty acids are transported across the cell membrane via a transport/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The fadL gene codes for a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transport. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assebled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
+Long-chain fatty acids are transported across the cell membrane via a transportation/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The fadL gene codes for a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transportation. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assembled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
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