Difference between revisions of "Part:BBa K785000"

Revision as of 05:47, 22 September 2012

Lov-tetR (light sensor with Ptet repression) light->PtetO repressed

This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033
Illegal AgeI site found at 478
1000
COMPATIBLE WITH RFC[1000]

Image of the structure of this protein

@@ Line 13: / Line 13: @@
 <partinfo>BBa_K785000 SequenceAndFeatures</partinfo>
-[[Image:Lov-TetR.png]]
+[[Image:Lov-TetR.png|frame|left|Image of the structure of this protein]]
 <!-- Uncomment this to enable Functional Parameter display