Difference between revisions of "Part:BBa K785000"

Revision as of 05:37, 22 September 2012

Lov-tetR (light sensor with Ptet repression) light->PtetO repressed

This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.

Sequence and Features