Difference between revisions of "Part:BBa K785000:Design"
(→‎Design Notes)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
 
This part is designed for our biowave project.
 
This part is designed for our biowave project.
−
See more about Biowave visit our wiki:[http://http://2012.igem.org/Team:Fudan_Lux link title]
+
See more about Biowave visit our wiki:[http://2012.igem.org/Team:Fudan_Lux Fudan_Lux 2012]
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 05:13, 22 September 2012

Lov-tetR (light sensor with Ptet repression) light->PtetO repressed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
    Illegal AgeI site found at 478
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for our biowave project. See more about Biowave visit our wiki:[http://2012.igem.org/Team:Fudan_Lux Fudan_Lux 2012]

Source

This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.

References