Difference between revisions of "Part:BBa K823040"

Revision as of 20:39, 21 September 2012

Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha

This part reverses the positive Input of the pBad Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha.

This Image depicts the ONPG assay done with this part. It converts the positive Input of the pBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 495
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K823040 short</partinfo>
-This part reverses the positive Input of the pBad Promoter induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha.
+This part reverses the positive Input of the pBad Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha.
-<!-- [[File:LMU Inverter graph|600px]]>
+[[Image:Inverter_graph.png|600px]]
+This Image depicts the ONPG assay done with this part. It converts the positive Input of the pBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).
+<!--