Difference between revisions of "Part:BBa K861024"
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<partinfo>BBa_K861024 short</partinfo>
 
<partinfo>BBa_K861024 short</partinfo>
  
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. FadE is originated from e.coli DH5&#945;, catalyzing the conversion of acyl-CoA to enoyl-CoA.
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. FadE is originated from e.coli DH5&#945;, catalyzing the conversion of acyl-CoA to enoyl-CoA.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:17, 21 September 2012

IPTG induced FadE

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. FadE is originated from e.coli DH5α, catalyzing the conversion of acyl-CoA to enoyl-CoA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 668
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 451
    Illegal SapI.rc site found at 1324
    Illegal SapI.rc site found at 2467