Difference between revisions of "Part:BBa K861037"
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<partinfo>BBa_K861037 short</partinfo>
 
<partinfo>BBa_K861037 short</partinfo>
  
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadI and FadJ.  
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadI and FadJ.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:16, 21 September 2012

IPTG induced FadI and FadJ

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadI and FadJ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]