Difference between revisions of "Part:BBa K861175"
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<partinfo>BBa_K861175 short</partinfo> | <partinfo>BBa_K861175 short</partinfo> | ||
| − | PI promoter contains the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Therefore, due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of the promoter. RFP was used to test the function of PI. | + | PI promoter contains the altered consensus CRP-binding site and the consensus RNA polymerase-binding site which have a overlap of several base pairs. Therefore, due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of the promoter. RFP was used to test the function of PI. |
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Latest revision as of 17:31, 21 September 2012
Efficacy testing RFP generator of BBa_K861170 (PI)
PI promoter contains the altered consensus CRP-binding site and the consensus RNA polymerase-binding site which have a overlap of several base pairs. Therefore, due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of the promoter. RFP was used to test the function of PI.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 617
Illegal AgeI site found at 729 - 1000COMPATIBLE WITH RFC[1000]