Difference between revisions of "Part:BBa K861176"

Revision as of 17:29, 21 September 2012

mRFP expressed after Pcar

Pcar promoter contains the consensus CRP-binding site and the altered RNA polymerase-binding site which have a overlap of several base pairs. Therefore, due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of the promoter. RFP was used to test the function of Pcar.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 617
Illegal AgeI site found at 729
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K861176 short</partinfo>
-PII is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.Therefore due to the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter.This generator contains PII and a screening plasmid intermediate designed by Josh.
+Pcar promoter contains the consensus CRP-binding site and the altered RNA polymerase-binding site which have a overlap of several base pairs. Therefore, due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of the promoter. RFP was used to test the function of Pcar.
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