Difference between revisions of "Part:BBa K861174"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K861174 short</partinfo>
 
<partinfo>BBa_K861174 short</partinfo>
  
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The promoter, J23113, was added to the cI coding piece without promoter, P0451. This should constitutively express the lambda repressor. Promoter lambda - cI regulated - (R0051) with RFP (E1010) as a reporter.  
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The promoter, BBa_J23113, was used to constitutively express cI protein (BBa_P0451). Promoter lambda - cI regulated (BBa_R0051) with RFP (BBa_E1010) was use as a reporter.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:22, 21 September 2012

A control device for BBa_K861169

The promoter, BBa_J23113, was used to constitutively express cI protein (BBa_P0451). Promoter lambda - cI regulated (BBa_R0051) with RFP (BBa_E1010) was use as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1611
    Illegal AgeI site found at 1723
  • 1000
    COMPATIBLE WITH RFC[1000]