Difference between revisions of "Part:BBa K784019"

Revision as of 16:43, 21 September 2012

Phage Lambda fragment 5 - recombination

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome.
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an E. coli cell (with the matching att-B sequence) between gal and bio operons.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 4318
Illegal BamHI site found at 543
Illegal XhoI site found at 6069
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823
Illegal AgeI site found at 4396
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K784019 short</partinfo>
-Hila, update this.
+This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
+Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br>
+Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an E. coli cell (with the matching att-B sequence) between gal and bio operons. <br>
 <!-- Add more about the biology of this part here