Difference between revisions of "Part:BBa K914008:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K914008 short</partinfo> | <partinfo>BBa_K914008 short</partinfo> | ||
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The system is leaky and meganuclease cut RS with high efficiency even without pRha induction. | The system is leaky and meganuclease cut RS with high efficiency even without pRha induction. | ||
| − | + | ===Sequence=== | |
| + | The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG) | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:24, 21 September 2012
Meganuclease I-SceI controlled by pRha
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The system is leaky and meganuclease cut RS with high efficiency even without pRha induction.
Sequence
The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)
Source
Promoter pRha was amplified from the plasmid pJOE3075 (Dr. Altenbuchner). RBS was taken from an iGEM distribution plate (BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).