Difference between revisions of "Part:BBa K914005:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K914005 short</partinfo> | <partinfo>BBa_K914005 short</partinfo> | ||
| Line 9: | Line 8: | ||
The pLac promoter is leaky. | The pLac promoter is leaky. | ||
| − | + | ===Sequence=== | |
| + | The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG) | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:23, 21 September 2012
Meganuclease I-SceI controlled by pLac
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pLac promoter is leaky.
Sequence
The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)
Source
Promoter pLac and RBS was taken from an iGEM distribution plate (BBa_R0011 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).