Difference between revisions of "Part:BBa K914006:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K914006 short</partinfo>
 
<partinfo>BBa_K914006 short</partinfo>
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It has very hight efficiency.
 
It has very hight efficiency.
  
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===Sequence===
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The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)
  
  

Revision as of 13:23, 21 September 2012

I-SceI meganuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has very hight efficiency.

Sequence

The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)


Source

Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

References