Difference between revisions of "Part:BBa K907004"

 
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__NOTOC__
 
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<partinfo>BBa_K907004 short</partinfo>
 
<partinfo>BBa_K907004 short</partinfo>
  
 
Dual phase protein generator(GFP default). mRFP and GFP
 
Dual phase protein generator(GFP default). mRFP and GFP
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This part is derivative of BBa_K907002.
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BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached both end of BBa_K907002 with overlapping extension PCR.
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This part generates GFP in E.coli(strain MG1655) normally.
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When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:15, 21 September 2012

Dual phase protein generator(GFP default). mRFP and GFP

Dual phase protein generator(GFP default). mRFP and GFP

This part is derivative of BBa_K907002.

BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached both end of BBa_K907002 with overlapping extension PCR. This part generates GFP in E.coli(strain MG1655) normally. When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 756
    Illegal NheI site found at 779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 838
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI.rc site found at 806
    Illegal BsaI.rc site found at 1499