Difference between revisions of "Part:BBa K907003"
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Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
 
Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
  
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This part is composed of 3 elements.  
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<br>This part is composed of 3 elements.  
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1. RBS: BBa_B0034, upsteam one is reversed  
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<br>1. RBS: BBa_B0034, upsteam one is reversed  
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2. Promoter: BBa_J23119, Bacterial constitutive promoter  
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<br>2. Promoter: BBa_J23119, Bacterial constitutive promoter  
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3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.
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<br>3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.
  
 
At the first state, this device promotes the transcription and translation of upstream reversed gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence between with attB and attP sequence, promoter orientation changed. Then this device promotes the transcription and translation of downstream gene.
 
At the first state, this device promotes the transcription and translation of upstream reversed gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence between with attB and attP sequence, promoter orientation changed. Then this device promotes the transcription and translation of downstream gene.

Revision as of 13:09, 21 September 2012

Binary signal generator, promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS

Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS


This part is composed of 3 elements.
1. RBS: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.

At the first state, this device promotes the transcription and translation of upstream reversed gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence between with attB and attP sequence, promoter orientation changed. Then this device promotes the transcription and translation of downstream gene.

This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 69
    Illegal NheI site found at 92
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 42
    Illegal BsaI.rc site found at 125