Difference between revisions of "Part:BBa K777002"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K777002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K777002 SequenceAndFeatures</partinfo>
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[[Image:K777002_in_pSB1C3.png|frame|left|'''Fig. 2:''' Plasmid map of K777002 in pSB1C3]]
  
 
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Revision as of 12:56, 21 September 2012

Tar receptor under the control of constitutive promoter J23104

Fig. 1: Overview of BioBricks containing the Tar gene downstream of Anderson promoters. (Activity according to Berkeley 2006)

The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.

Here we used a set of 8 constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive Tar expression affect the chemotaxis of E. coli.












Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 152


Fig. 2: Plasmid map of K777002 in pSB1C3