Difference between revisions of "Part:BBa K802003:Design"
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The SpeI site from the B. subtilis coding region of the pHT304 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector. | The SpeI site from the B. subtilis coding region of the pHT304 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector. | ||
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===Source=== | ===Source=== | ||
Revision as of 12:15, 21 September 2012
Shuttle vector for E. coli and B. subtilis
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 6506
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6506
Illegal NheI site found at 3377
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6512 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6506
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 6506
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 6506
Illegal XbaI site found at 6521
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3203
Illegal BsaI.rc site found at 5190
Design Notes
The SpeI site from the B. subtilis coding region of the pHT304 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector.
<img src="
" width=150px"/>
Source
The shuttle vector is derived from pHT304 (Arantes & Lereclus, 1991). It is a low copy plasmid number in B. subtilis.