Difference between revisions of "Part:BBa K914007:Design"
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<partinfo>BBa_K914007 short</partinfo> | <partinfo>BBa_K914007 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
The system is leaky and meganuclease cut RS with high efficiency even without pBad induction. | The system is leaky and meganuclease cut RS with high efficiency even without pBad induction. | ||
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| + | ===Sequence=== | ||
| + | The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG) | ||
Latest revision as of 09:49, 21 September 2012
Meganuclease I-SceI controlled by pBad
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The system is leaky and meganuclease cut RS with high efficiency even without pBad induction.
Sequence
The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)
Source
Promoter pBad and RBS was taken from an iGEM distribution plate (BBa_I13453 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).