Difference between revisions of "Part:BBa K861171"
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<partinfo>BBa_K861171 short</partinfo> | <partinfo>BBa_K861171 short</partinfo> | ||
| − | This is a promoter designed for the ''Eschaerichia coli''. It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells, low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes | + | This is a promoter designed for the ''Eschaerichia coli''. It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells, low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. |
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Revision as of 06:57, 21 September 2012
Pcar, synthetic promoter repressed by CRP
This is a promoter designed for the Eschaerichia coli. It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells, low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]