Difference between revisions of "Part:BBa K861177"
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<partinfo>BBa_K861177 short</partinfo> | <partinfo>BBa_K861177 short</partinfo> | ||
| − | + | To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadD. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 06:02, 21 September 2012
K861162+ K861175
To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 815 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1425
Illegal AgeI site found at 1537 - 1000COMPATIBLE WITH RFC[1000]