Difference between revisions of "BBa K731700 and BBa K731710 measurements"
Line 5: Line 5:
 
The develop of this protocolcost us not only sleepless night but also sunny and beautiful weekend of trekking so just shut up and read it all.
 
The develop of this protocolcost us not only sleepless night but also sunny and beautiful weekend of trekking so just shut up and read it all.
  
−
{| width=50% style='border: 2px solid red'
+
{|width='80%'
−
|-
+
−
|'''Using primers VR/VF2 to PCR B0010 will result in excess bands.'''
+
−
'''See a full description of the problem [[Problems with PCR using VR/VF2|here]].'''
+
 
|-
 
|-
 +
|width=35% valign='top'|
 +
[[Image:Terminator.png]]
 +
<partinfo>BBa_B0010 parameters</partinfo>
 +
| width=50% valign='top' style='border: 1px solid black'|
 +
<partinfo>BBa_B0010 short</partinfo>
 +
* Transcriptional terminator consisting of a 64 bp stem-loop.
 +
 
|}
 
|}
  
 
Measurements of part K731700 and K731710
 
Measurements of part K731700 and K731710

Revision as of 20:37, 20 September 2012

Hello world

In this page we are really proud to introduce you the protocol we (Giacomo and Anna) developed for the characterization transcriptional terminators effects on gene expression. (Tested on BBa_J64997)

The develop of this protocolcost us not only sleepless night but also sunny and beautiful weekend of trekking so just shut up and read it all.

Terminator.png

biologyrrnBT1
directionForward
forward_efficiency-NA-
reversed_version582
reverse_efficiency-NA-

T1 from E. coli rrnB

  • Transcriptional terminator consisting of a 64 bp stem-loop.

Measurements of part K731700 and K731710