Difference between revisions of "Part:BBa K743001:Design"
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According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size. | According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size. | ||
| − | + | This part has a EcoR1 site at position 132 wich wasn´t mutated so that there´s complete homology with Synechocystis chromosome region. The restricion site should´t be a problem for standard assemblies as long as this part is used as a downstream brick. | |
===Source=== | ===Source=== | ||
Latest revision as of 19:09, 20 September 2012
Synechocystis PCC6803 neutral recombination site.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 132
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 132
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 132
Illegal XhoI site found at 629 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 132
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 132
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
This part has a EcoR1 site at position 132 wich wasn´t mutated so that there´s complete homology with Synechocystis chromosome region. The restricion site should´t be a problem for standard assemblies as long as this part is used as a downstream brick.
Source
PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337