Difference between revisions of "Part:BBa K743000:Design"
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===Source=== | ===Source=== | ||
| − | PCR amplified from synechocystis PCC6803 chromosome. | + | PCR amplified from synechocystis PCC6803 chromosome, orf slr0370. |
===References=== | ===References=== | ||
Revision as of 14:50, 20 September 2012
Synechocystis PCC6803 neutral recombination site.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
Source
PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.