Difference between revisions of "Part:BBa K818500:Design"
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<partinfo>BBa_K818500 short</partinfo> | <partinfo>BBa_K818500 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | The regulator sboA is derived from B. subtilis so this coding construct will work in B. subtilis. | + | The regulator sboA is derived from B. subtilis so this coding construct will work in B. subtilis. |
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| + | ===Design Notes Regarding Cloning Strategy=== | ||
| + | The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy. | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:23, 20 September 2012
Lycopene with sboA regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 207
Illegal BamHI site found at 2242 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1778
Illegal NgoMIV site found at 1908
Illegal AgeI site found at 993 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The regulator sboA is derived from B. subtilis so this coding construct will work in B. subtilis.
Design Notes Regarding Cloning Strategy
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
Source
This part is combined from biobricks: BBa_K818100 and BBa_K274200.