Difference between revisions of "Part:BBa K818400:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | BBa_K818100 is a biobrick of Bacillus subtilis regulator that reacts to rotten meat volatiles | + | BBa_K818100 is a biobrick of Bacillus subtilis regulator that reacts to rotten meat volatiles. This construct works best in B. subtilis. |
===Design Notes Regarding Cloning Strategy=== | ===Design Notes Regarding Cloning Strategy=== | ||
Latest revision as of 13:20, 20 September 2012
AmilCP with sboA regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa_K818100 is a biobrick of Bacillus subtilis regulator that reacts to rotten meat volatiles. This construct works best in B. subtilis.
Design Notes Regarding Cloning Strategy
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
Source
This part is a combination of parts from biobricks: BBa_K818100 and BBa_K592025