Difference between revisions of "Part:BBa K818400:Design"
Emeraldo88 (Talk | contribs) (→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K818400 short</partinfo> | <partinfo>BBa_K818400 short</partinfo> | ||
| Line 9: | Line 8: | ||
BBa_K818100 is a biobrick of Bacillus subtilis regulator that reacts to rotten meat volatiles, so this construct would work best in B. subtilis therefore, a supporting backbone is needed. | BBa_K818100 is a biobrick of Bacillus subtilis regulator that reacts to rotten meat volatiles, so this construct would work best in B. subtilis therefore, a supporting backbone is needed. | ||
| − | + | ===Design Notes Regarding Cloning Strategy=== | |
| + | The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy. | ||
===Source=== | ===Source=== | ||
Revision as of 13:17, 20 September 2012
AmilCP with sboA regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa_K818100 is a biobrick of Bacillus subtilis regulator that reacts to rotten meat volatiles, so this construct would work best in B. subtilis therefore, a supporting backbone is needed.
Design Notes Regarding Cloning Strategy
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
Source
This part is a combination of parts from biobricks: BBa_K818100 and BBa_K592025